Effects of Amantadine on Liver and Lung Tissue in Hepatic Ischemia Reperfusion Injury in Rats

Authors

  • Fatih Şahin Sakarya University Training and Research Hospital
  • Ayca Tas Tuna Sakarya University, Faculty of Medicine
  • Yusuf Ünal Gazi University, Faculty of Medicine
  • Mustafa Arslan Gazi University, Faculty of Medicine
  • Hayrullah Yazar Sakarya University, Faculty of Medicine
  • Şaban Cem Sezen Kirikkale University, Faculty of Medicine
  • Sezen Irmak Gözükara Sakarya University Training and Research Hospital
  • Onur Palabıyık Sakarya University, Faculty of Medicine

Abstract

Background: N-Methyl D-Aspartate (NMDA) receptor blockers have been shown to have protective effects against ischemia/reperfusion (I/R) injury in various tissues. The aim of this study was to investigate the effects of amantadine on liver and lung tissue in hepatic I/R injury.

Materials and Methods: Twenty-four rats divided into 4 groups: the Sham Group (S), the Amantadine Group (A), the I/R Group (I/R) and the I/R + Amantadine Group (I/R-A). In Group A and Group I/R-A, 45 mg/kg of amantadine was administered before surgery. In Group I/R and Group I/R-A, an atraumatic vascular clamp was applied to the structures in the left portal triad for 45 minutes and reperfusion period was 2 hours after declampage. Malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) enzyme levels were were studied in liver and lung tissues. Additionally tissues were examined histopathologically.

Results: No significant difference was observed in tissue MDA, SOD, and CAT levels among four groups (p >0.05). Polymorphonuclear cell infiltration and the scores of hepatocyte degeneration, sinusoidal dilatation, pycnotic core, and necrosis cell were significantly higher in Group I/R than other groups (p<0.05). Regarding to the lung tissue, the neutrophil/lymphocyte infiltration score was significantly lower in Group S and A than in Group I/R (respectively; p= 0.007, 0.011), and it was significantly higher in Group I/R-A than in Group S (p = 0.014). The alveolar wall thickening score was significantly higher in Group I/R than the other groups (p <0.0001).

Conclusion: Amantadine may have a protective effect against I/R damage, as it reduces histopathological changes caused by I/R damage.

Author Biographies

Ayca Tas Tuna, Sakarya University, Faculty of Medicine

Department of Anesthesiology

Yusuf Ünal, Gazi University, Faculty of Medicine

Department of Anesthesiology

Mustafa Arslan, Gazi University, Faculty of Medicine

Department of Anesthesiology,

Hayrullah Yazar, Sakarya University, Faculty of Medicine

Department of Biochemistry

Şaban Cem Sezen, Kirikkale University, Faculty of Medicine

Department of Histology and Embryology

Sezen Irmak Gözükara, Sakarya University Training and Research Hospital

Department of Biochemistry

Onur Palabıyık, Sakarya University, Faculty of Medicine

Department of Anesthesiology

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Published

08.12.2022

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Section

Original Research