Dual Therapy with Ellagic Acid and Carnosic Acid Ameliorates STZ-Induced Diabetic Conditions in Rats by Alleviating Liver Oxidative Stress

Ellagic Acid, Carnosic Acid and Liver Oxidative Stress in Diabetic Rats

Abstract

Objective: Diabetes, a serious public health issue today, causes serious health problems with its complications and will become a global epidemic if no action is taken. Diabetic patients experience elevated oxidative stress, which impairs wound healing. The purpose of this study was to look into the effects of ellagic acid (EA) and carnosic acid (CA) on oxidative events in liver tissue in diabetic rats. Methods: The rats were divided into 4 main groups as control, untreated, topical and oral treatment group. To develop diabetes, each group received a single intraperitoneal injection of streptozotocin (STZ). Wounds were treated with EA and CA in a carrier gel by both topically or orally. On the 3rd and 7th day of recovery, the rats were sacrificed and the levels of nitric oxide (NOx), malondialdehyde (MDA), glutathione (GSH), protein carbonyls (PC), ascorbic acid (AA) were measured spectrophotometrically to investigate the effects of oxidative stress in liver tissue. Results: Liver tissue MDA, NOx and PC levels were determined to be statistically decreased in both topical and oral applications compared to the control and untreated groups (p<0.05). Liver tissue GSH, AA and collagen levels were found to be statistically increased in both topical and oral applications when compared to the control and untreated groups (p<0.05). Conclusion: The combination of EA and CA with two different application methods seems to have an impact on reducing oxidative stress in liver tissue, hopefully preventing possible complications of diabetes at an early stage.