The Effect of Hydrogen-Rich Saline Solution on Erythrocyte Deformability in Lower Limb Ischemia Reperfusion Injury in Rats
Abstract
Aim: We aimed to investigate the effects of HRSS on erythrocyte deformability in lower limb ischemia reperfusion (IR) injury in rats.
Methods: Eighteen male Wistar albino rats used in this study. The animals were randomly divided into three experimental groups as control, IR and IR-HRSS. 20 mg.kg-1HRSS was administered (20 mg.kg-1 i.p)30 min before the procedure. An atraumatic microvascular clamp was placed across the infrarenal abdominal aorta in the IR groups. 120 min ischemia and 120 min reperfusion is applied to the groups. Erythrocytes were obtained from heparinized whole blood samples for deformability measurements. Kruskal-Wallis test was used for independent samples and Mann-Whitney U test was used to analyze differences between groups.
Results: Ischemia reperfusion was found to increase relative resistance to the control group. The erythrocyte deformability index was significantly higher in IR and IR-HRSS groups than the control group. HRSS application significantly decreased erythrocyte deformability index compared to IR group.
Conclusion: IR induced rats decreased erythrocyte deformability was partially corrected by HRSS. We believe that the protective effects of HRSS in IR injury and its use indications can be demonstrated in detail as long as the findings we have reached in our study are supported by other studies.
References
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