Adrenomedullin Prevents Lung Injury after Hepatic Ischemia-Reperfusion Damage

  • Kürşat Dikmen Gazi University
  • Hasan Bostanci Gazi University
  • Aydin Yavuz Yildirim Beyazit University
  • Huseyin Gobut Yildirim Beyazit University
  • Murat Alper Diskapi Yildirim Bayezit Training and Education Hospital
  • Mustafa Kerem Gazi University
Keywords: Adrenomedullin, hepatic ischemia/reperfusion, lung injury


Objective: Acute lung injury is a complication after hepatic ischemia/reperfusion (I/R) and might be responsible for high mortality rate. The aim of this study was to demonstrate that adrenomedulline administration may prevent lung injury after hepatic I / R by downregulation of proinflammatory cytokines. Materials and Methods: This study was performed by using 54 male Wistar rats. The rats were randomly allocated into 3 groups and in groups were randomly allocated into 1st, 2nd, and 4th hour subgroups. After I/R, AM (12μg/kg) was infused for 30 minute via portal vein. Blood and tissue samples were collected 1, 2 and 4 hour after reperfusion. Hepatic I/R induced lung injury, as characterized by lung edema, histopathologic changes and proinflammatory cytokines including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were evaluated. Results: The TNF-α enzyme activity in the first hour was found to be significantly higher in the I/R group than in the Sham and AM group (p=0.027 and p=0.041, respectively). The levels of TNF-α in the sham-operated group were similar in the AM group (p=0.310). Significant differences were found only in the second hour IL-6 measurements (p=0.038). Similar differences in caspase-9 enzyme activity in the sham-operated and AM group (p=0.291). Treatment with AM decreased lung injury after hepatic I/R as demonstrated by reduction in histologic damage. Conclusions: Acute lung injury was decreased by AM treatment after hepatic I/R injury.

Author Biographies

Kürşat Dikmen, Gazi University
Department of General Surgery
Hasan Bostanci, Gazi University
Department of General Surgery
Aydin Yavuz, Yildirim Beyazit University
Department of General Surgery
Huseyin Gobut, Yildirim Beyazit University
Department of General Surgery
Murat Alper, Diskapi Yildirim Bayezit Training and Education Hospital
Department of Pathology
Mustafa Kerem, Gazi University
Department of General Surgery


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Original Research