The Effects of Taurine, Melatonin and N-Acetylcystein on Cadmium Exposure Bone Changes “The Surprising Effect of Taurine”
Abstract
Background: Chronic environmental and occupational exposure to cadmium can result in skeletal system changes. The main objective of the present study was to investigate and compare the effects of taurine, melatonin and N-acetyl cysteine on cadmium exposure induced bone density loss.
Methods: 90 adult male Sprague-Dawley rats were allocated into four main groups: Group I was the control group; Group II was the “cadmium exposure” group; Group 3 was “cadmium exposure for 3 months + concurrent antioxidant administration” group. The concept of Group 4 was cadmium exposure for 3 months + subsequent antioxidant administration. Bone mineral density values were evaluated in all the groups. Serum calcium, phosphorus, alkaline phosphatase (ALP) enzyme activities and 24 hours urine calcium excretion levels were measured. Kruskal–Wallis test was used to compare the all groups. Between two group comparisons, the Mann–Whitney U test was used.
Results: There was no significant difference in terms of bone mineral density values only between control group and cadmium exposure group (p>0.05). Mean bone mineral density values obtained in “cadmium + concurrent taurine” and “cadmium + subsequent taurine” groups were significantly lower than all the other groups (p<0.05). 24 hours urine calcium excretion levels were significantly higher in groups which taurine and n-asetylcystein were administered after cadmium exposure.
Conclusion: Taurine, which is thought to have protective effects as an antioxidant caused a marked bone damage after exposure to cadmium. Further studies are needed to clarify this effect of taurine.
References
REFERENCES
Åkesson A., Bjellerup P., Lundh T, Lidfeldt J et al.: Cadmium-induced effects on bone in a population-based study of women. Environ Health Perspect, 2006, 114(6), 830–834.
Aydogdu N, Kanter M, Erbaş H, Kaymak K: The effects of taurin, melatonin and acetylcysteine on nitric oxide, lipid peroxidation and some antioxidants in cadmium induced liver injury. Erciyes Med Journal 2007, 29(2), 89-96.
Baldwin DR, Marshall WJ: Heavy metal poisoning and its laboratory investigation. Ann Clin Biochem, 1999, 36, 267-300.
Brzóska MM, Moniuszko-Jakoniuk J: Low-level lifetime exposure to cadmium decreases skeletal mineralization and enhances bone loss in aged rats. Bone, 2004, 35(5), 1180-1191.
Choi MJ: Effects of taurine supplementation on bone mineral density in ovariectomized rats fed calcium deficient diet. Nutr Res Pract, 2009, 3(2), 108-113.
Choi MJ, Di Marco NM: The effects of dietary taurine supplementation on bone mineral density in ovariectomized rats. Adv Exp Med Biol, 2009, 643, 341-349.
Doi T, Puri P, Bannigan J, Thompson J: Pre-treatment with N-acetylcysteine upregulates superoxide dismutase 2 and catalase genes in cadmium-induced oxidative stress in the chick omphalocele model. Pediatr Surg Int, 2011, 27(2), 131-136.
Feldman SL, Cousins RG: Influence of cadmium on the metabolism of 25-hydroxycholecalciferol in chicks. Nutr Rep Int, 1973, 8, 251-259.
Fernández-Pérez B, Caride A, Cabaleiro T, Lafuente A: Cadmium effects on 24h changes in glutamate, aspartate, glutamine, GABA and taurine content of rat striatum. J Trace Elem Med Biol, 2010, 24(3), 212-218.
Goyer RA: Toxic effects of metals. In: Klaassen, C.D. (Ed.), Casarett & Doull’s Toxicology: The Basic Science of Poisons, fifth ed. McGraw-Hill, New York, 1996.
Hashimoto K, Pinkas G, Evans L, Liu H, Al-Hasan Y, Thompson LP: Protective effect of N-acetylcysteine on liver damage during chronic intrauterine hypoxia in fetal guinea pig. Reprod Sci, 2012, 19(9), 1001-1009.
International Programme on Chemical Safety. Cadmium-Environmental Health Criteria 134. Geneva: World Health Organization. Available http://www.inchem.org/documents/ehc/ehc/ehc134.htm.
Kaplan M, Atakan IH, Aydoğdu N, Aktoz T, et al: Influence of N-acetylcysteine on renal toxicity of cadmium in rats. Pediatr Nephrol, 2008, 23(2), 233-241.
Karabulut-Bulan Ö, Koyutürk M, Bolkent F. et al: Sıçan tiroid bezinde kadmiyum hasarına karşı C vitamini, E vitamini ve selenyumun kombine kullanımının etkileri. Cerrahpaşa Tıp Dergisi 2004, 35, 174-180.
Manna P, Sinha M, Sil PC: Taurine plays a beneficial role against cadmium-induced oxidative renal dysfunction. Amino Acids, 2009, 36(3), 417-428.
Nakamura T, Ogasawara M, Koyama I, Nemoto M, et al: The protective effect of taurine on the biomembrane against damage produced by oxygen radicals. Biol Pharm Bull 1993, 16, 970-972.
Nordberg M, Jin T, Nordberg GF: Cadmium, metallothionein and renal tubular toxicity. IARC Sci. Publ 1992; 118: 293-297.
Patrick L. Toxic metals and antioxidants: Part II. The role of antioxidants in arsenic and cadmium toxicity. Altern Med Rev, 2003, 8(2), 106-128.
Rignell-Hydbom A, Skerfving S, Lundh T, Lindh CH et al: Exposure to cadmium and persistent organochlorine pollutants and its association with bone mineral density and markers of bone metabolism on postmenopausal women. Environ Res 2009, 109(8), 991-996.
Reiter RJ, Tan DX, Burkhardt S: Reactive oxygen and nitrogen species and cellular and organismal decline: amelioration with melatonin. Mech Ageing De, 2002 123, 1007-1119.
Sinha M, Manna P, Sil PC: Induction of necrosis in cadmium-induced hepatic oxidative stress and its prevention by the prophylactic properties of taurine. J Trace Elem Med Biol, 2009, 23(4), 300-313.
Sinha M, Manna P, Sil PC: Taurine protects the antioxidant defense system in the erythrocytes of cadmium treated mice. BMB Rep, 2008,; 41(9), 657-663.
Zeneli L, Daci N, Paçarizi H, Daci-Ajvazi M: Interaction between Cadmium and Calcium in Human Blood at the Smokers. Am J Pharm & Toxicol, 2010, 5(1), 48-51.
