Evaluation of C-Reactive Protein in Iraqi Children Presented with Acute Enteropathogenic Escherichia Coli Associated Diarrhea with Special Emphasis to Age and Gender
Abstract
Background and Objectives: The aim is to determine the role of Enteropathogenic Escherichia coli (EPEC) serotypes (O111, O55, O26, O86, O119, O127, O114, and O142) in C- reactive protein (CRP) level in acute diarrhea among children; to determine the possible effect of age and gender in CRP level.
Methods: Culture of stool samples from 64 children in MacConkey agar for primary isolation of E.coli and confirmed via API20E . Sero-grouping of E.coli performed via specific antisera . CRP detected by latex agglutination.
Results: In this study, 64 children with diarrhea were involved. The mean age was 2.92 years. Males represent 34/64, 53.1%. The range of CRP level 12-96 mg/dl. EPEC1 and EPEC3 not detected. EPEC2 detected among 14/64, 21.9% and EPEC4 in 18.8%. Among the age group ˂1 year, EPEC2 detected in 9.38%, with mean CRP level 12mg/dl; 3.13%, 48 mg/dl 6.25% . EPEC4 detected in 2/64, 3.13%, with mean CRP level 12mg/dl. Age group 1-4 year, EPEC2 detected in 6/64 , 9.38% , with mean CRP level 24mg/dl. EPEC4 detected in 6/64, 9.38%; with mean CRP level 12mg/dl, 24mg/dl, 48mg/dl 3.13% in each. Among the age group 4.1-8 year, EPEC4 detected in 4/64 ,6.25% , with CRP mean level 48 mg/dl. EPEC not detected in 8˃ years age group .EPEC2 detected in male 28/64 , 43.75% and the CRP level range from 12-96mg/dl .EPEC2 detected in 22/64 females, 34.38% , CRP level range from 12-96mg/dl . No significant difference or correlation between gender and CRP level detected. EPEC4 detected in males 26/64 , 40.63% , CRP level range from 12-96mg/dl . EPEC4 detected in 26/64 females , 40.63% , CRP level range from 12-96mg/dl . No significant difference or correlation between gender and CRP level was detected. EPEC2 and CRP level significantly correlated with patients age . while infection with EPEC2 inversely correlated EPEC4. No significant correlation between gender and EPEC serotypes.
Conclusion: Although EPEC2 and EPEC4 frequently detected in diarrhea cases of 1-8 years old, only EPEC2 positively correlated with age. Even with a fluctuation in CRP level, it was positively correlated with age and not correlated with EPEC serotypes and gender.
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