Investigation of Aflatoxin B1 Levels in Red-scaled Pepper by ELISA

Authors

  • Sezen Güntekin Ergün
  • Semiha Özkan
  • Ufuk Abbasoğlu

Abstract

Objective: Mycotoxins have carcinogenic, mutagenic and teratogenic effects on most animals and humans. Among all mycotoxins, aflatoxin B1 (AFB1) has the most toxic effects.

Methods: In this study, the aflatoxin B1 levels in red-scaled pepper samples have been determined by ELISA method.

Results: The results revealed that 69 out of 82 samples (84.14%) were found to be contaminated with aflatoxin B1 in the range of 5.1-20.94 μg kg-1 (ppb).

Conclusion: Aflatoxins are dangerous contaminants of red pepper and have to be considered as a major concern for public health. 

References

-Colak, H., Bingol, E.B., Hampikyan, H., et al. Determination of aflatoxin contamination in red scaled, red and black pepper by ELISA and HPLC. Journal of Food and Drug Analysis 2006;14:292-6.

-Farombi, E.O. Aflatoxin contamination of foods in developing countries: Implications for hepatocellular carcinoma and chemopreventive strategies. African Journal of Biotechnology 2006;5:1-14.

-Bennett, J.W., Klich, M. Mycotoxins. Clinical Microbiology Reviews 2003;16:497-516.

-Diaz, G.J., Espitia, E. Occurrence of aflatoxin M1 in retail milk samples from Bogotá, Colombia. Food Additives and Contaminants 2006;23:811-5.

-Unusan, N. Occurence of aflatoxin M1 in 347 UHT milk in Turkey. Food and Chemical Toxicology 2006;44:1897-900.

-Blesa, J., Soriano, J.M., Molto, J.C., et al. Limited survey for the presence of aflatoxins in foods from local markets and supermarkets in Valencia, Spain. Food Additives and Contaminants 2004;21:165-71.

-Martins, M.L., Martins, H.M., Bernardo, F. Aflatoxins in spices marketed in Portugal. Food Additives and Contaminants 2001;18: 315-9.

-Turkish Food Codex. 2008. The Maximum Allowed Level of Food Contaminants. Regulation No. 2008/26, Ankara.

-European Commission. Comission Regulation. 2006. 279 1881/2006 of the European parliament and the council of 19 December 2006 on the setting maximum levels for certain contaminants in food stuffs. Official Journal of the European Union L 364/5.

-Hu, Y.Y., Zheng, P., Zhang, Z.X., et al. Determination of aflatoxins in high pigment content samples by matrix solid-phase dispersion and high-performance liquid chromatography. Journal of Agricultural and Food Chemistry 2006;14: 4126-30.

-Sun, G., Wang., S, Hu, X., et al. Co-contamination of aflatoxin B1 and fumonisin B1 in food and human dietary exposure in three areas of China. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2011;28:461-70.

-Agaoglu, S. Van ilinde acikta satilan kirmizi pul biberlerde aflatoksin B1 varliginin arastirilmasi. Van Tip Dergisi 1999;6:28-30.

-Erdogan, A. The aflatoxin contamination of some pepper types sold in Turkey. Chemosphere 2004;56:321-5.

-Kursun, O., Mutlu, A.G. Aflatoxin in spices marketed in west mediterrenian region of Turkey. Journal of Animal and Veterinary Advances 2010;9: 2979-81.

-Fazekas, B., Tar, A., Kovacs, M. Aflatoxin and ochratoxin A content of spices in Hungary. Food Additives and Contaminants 2005;22:856-63.

-Fufa, H., Urga, K. Screening of aflatoxins in Shiro and ground red pepper in Addis Ababa. Ethiopian Medical Journal 1996;34:243-9.

-Reddy, K.R.N., Farhana, N.I., Salleh, B. Occurrence of Aspergillus spp. and Aflatoxin B1 in Malaysian Foods Used for Human Consumption”. Journal of Food Science 2011;76: 99-104.

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Published

30.09.2016

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Original Research