Protective Effects of N-Acetyl Cysteine against Paclitaxel-Induced Cardiotoxicity Through Modulation of Transient Receptor Potential Melastatin 2 Channels

  • Can Ramazan Öncel Alanya Alaaddin Keykubat University , School of Medicine
  • Ishak Suat Ovey Alanya Alaaddin Keykubat University , School of Medicine
Keywords: Apoptosis, paclitaxel, cardiomyocyte, transient receptor potential melastatin 2, N-acetyl cysteine

Abstract

Aim : In our study ,we investigated the paclitaxel induced cardiotoxicity and alterations in Ca2+ influx , oxidative stress and apoptosis through transient receptor potential melastatin 2 (TRPM2) channels and modulator role of N-acetyl cysteine (NAC) in cardiomyocytes. Material and Methods : All cells were cultured at 37°C. The cells were divided into seven main groups. Cells in the paclitaxel group were incubated with 2.5 μM Paclitaxel for 12 hours and cells in the NAC+Paclitaxel group were incubated with 2.5 μM Paclitaxel for 12 hours and then incubated with 10 μM NAC for 24 hours. Intracellular free calcium concentration , reactive oxygen species (ROS) production measurements and cell viability analyses were done according to the study protocol. Results : Cytosolic calcium levels, apoptosis levels, intracellular ROS production levels were lower in paclitaxel+NAC group than in the paclitaxel group of cardiomyocytes. Also values were markedly lower in the paclitaxel+NAC+antranilic acid group when compared to the paclitaxel+NAC group. Conclusion : We found that TRPM2 channels are overactivated during paclitaxel induced cardiotoxicity and NAC could show a cardioprotective effect through TRPM2 channel modulation.

Author Biographies

Can Ramazan Öncel, Alanya Alaaddin Keykubat University , School of Medicine
Department of Cardiology
Ishak Suat Ovey, Alanya Alaaddin Keykubat University , School of Medicine
Department of Physiology

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Published
2019-03-25
Section
Original Research