Protective Effects of Resveratrol in a Rat Model of Ischemia-Reperfusion Injury of Sciatic Nerve

  • Onur Akarca
  • Özgür Öcal
  • Erkut Baha Bulduk department of neurosurgery
  • Özge Tuğçe Paşaoğlu
  • Ayşe Çakır Gündoğdu
  • Gülnur Take Kaplanoğlu
  • Aydın Paşaoğlu
Keywords: Sciatic nerve, ischemia- reperfusion injury, resveratrol, rat, nitric oxide, malondialdehyde

Abstract

Objective: The aim of this study was to determine the effects of resveratrol on ischemia- reperfusion (I/R) injury in the rats sciatic nerve. Methods: We used 18 Wistar rats anesthetized with ketamine (50 mg/kg-im). In the sham group, we only performed the surgery and not administer any medication. In the ischemia group, we generated I/R injury in 5 minute intervals with medical treatment of saline only. In the resveratrol group, we brought about the same I/R injury but gave resveratrol before the surgery by per oral. After sacrifice, we studied blood and nerve tissue samples. We evaluated the damage with malondialdehyde (MDA), nitric oxide (NO), and total antioxidant capacity (TAC), both in tissue and blood. Results: Evaluation of serum levels of antioxidant markers and tissue samples demonstrated statistically significant effects of resveratrol in ischemia-reperfusion injury. Conclusion: This study revealed the antioxidant effect of resveratrol as an inhibitor of lipid peroxidation.

References

Joseph E, and Schoepfle GM. A study of nerve degeneration and regeneration. American Journal of Physiology-Legacy Content. 1946;147: 550-81.

Weisfeldt ML. Reperfusion and reperfusion injury. Clin Res. 1987; 35: 13-20.

Greene HL, Weisfeldt ML. Determinants of hypoxic and post-hypoxic myocardial contracture. Am J Physiol. 1997; 232 (5): 526-33.

Mahboob M, Siddiqui M.K. Long-term effects of a novel phosphorothionate (RPR- II) on detoxifying enzymes in brain, lung and kidney rats. Ecotoxicol Environ Saf. 2002; 53: 355-60.

Ramón y Cajal S. The structure and connexions of neurons. 1906 Nobel prize acceptance speech.

Ménard C, Stéphane B, and Quirion R. Neuroprotective effects of resveratrol and epigallocatechin gallate polyphenols are mediated by the activation of protein kinase C gamma. Front Cell Neurosci. 2013; 7: 281.

Marchal J, Pifferi F, and Aujard F. Resveratrol in mammals: effects on aging biomarkers, age‐related diseases, and life span. Annals of the New York Academy of Sciences. 2013; 1290: 67-73.

Leonard SS, Xia C, Jiang BH, Stinefelt B, Klandorf H, Harris GK, Shi X. Resveratrol scavenges reactive oxygen species and effects radical-induced cellular responses. Biochemical and biophysical research communications. 2003; 309:1017-26.

Takayuki S, Nakazato T, Xian MJ, Sagawa M, Ikeda Y, Kizaki M. Resveratrol induces apoptosis of human malignant B cells by activation of caspase-3 and p38 MAP kinase pathways. Biochemical pharmacology. 2006; 71: 742-50.

Tsai S, Hung L, Fu Y, Cheng H, Nien M, Liu H, Zhang F, Huang S. Resveratrol neuroprotective effects during focal cerebral ischemia injury via nitric oxide mechanism in rats. Journal of vascular surgery. 2007; 46: 346-53.

Huang, S.S., Tsai, M.C., Chih, C.L., Hung, L.M. and Tsai, S.K.Resveratrol reduction of infarct size in Long-Evans rats subjected to focal cerebral ischemia. Life Sci.2001; 69: 1057-65.

Hung LM, Chen JK, Huang SS, Lee RS, and Su MJ. Cardioprotective effect of resveratrol, a natural antioxidant derived from grape. Cardiovasc Res. 2000; 47: 549-55.

Fremont L. Biological effects to resveratrol. Life Sci 2000; 66: 663-73.

Shigematsu S, Ishida S, Hara M, Takahashi N, Yoshimatsu H, Sakata T, Korthuis RJ. Resveratrol, a red wine constituent polyphenol, prevents superoxide-dependent inflammatory responses induced by ischemia/reperfusion, platelet-activating factor, or oxidants. Free Radic Biol Med. 2003; 34: 810-7.

Liao YF, Zhu W, Li DP, and Zhu X. Heme oxygenase-1 and gut ischemia/reperfusion injury: a short review. World J Gastroenterol. 2013; 19: 3555-61.

Weyker PD, Webb CAJ, Kiamanesh D, and Flynn BC. Lung ischemia reperfusion injury: a bench-to-bedside review. Semin Cardiothorac and Vasc Anesth. 2013; 17: 28-43.

Vardanian AJ, Busuttil RW, and Kupiec-Weglinski JW. Molecular mediators of liver ischemia and reperfusion injury: a brief review. Molec Med. 2008; 14: 337-45.

Antonuccio P, Minutoli L, Romeo C, Nicotina PA, Bitto A, Arena S, Altavilla D, Zuccarello B, Polito F, Squadrito F. Lipid peroxidation activates mitogen-activated protein kinases in testicular ischemia-reperfusion injury. J Urol. 2006; 176: 1666-72.

Yaylak F, Canbaz H, Caglikulekci M, Dirlik M, Tamer L, Ogetman Z, Polat Y, Kanik A, Aydin S. Liver tissue inducible nitric oxide synthase (iNOS) expression and lipid peroxidation in experimental hepatic ischemia reperfusion injury stimulated with lipopolysaccharide: the role of aminoguanidine. J Surg Res. 2008; 148: 214-23.

Folino A, Losano G, and Rastaldo R. Balance of nitric oxide and reactive oxygen species in myocardial reperfusion injury and protection. J Cardiovasc Pharmacol. 2013; 62: 567-75.

Abu-Amara M, Yang SY, Seifalian A, Davidson B, and Fuller B. The nitric oxide pathway–evidence and mechanisms for protection against liver ischaemia reperfusion injury. Liver Int. 2012; 32:531-43.

Dong W, Li F, Pan Z, Liu S, Yu H, Wang X, Bi S, Zhang W. Resveratrol ameliorates subacute intestinal ischemia-reperfusion. J Surg Res. 2013; 185: 182-89.

Sim˜ao F, Matt´e A, Matt´e C, Soares FMS, Wyse ATS, Netto CA, Salbego CG. Resveratrol prevents oxidative stress and inhibition of Na+K+-ATPase activity induced by transient global cerebral ischemia in rats. J Nutr Bioch. 2011; 22: 921-28.

Liu F, Tsai H, Yu H. Organ protective effects of red wine extract, Resveratrol, in oxidative stres-mediated reperfusion injury. Oxidative Medicine and Cellular Longevity. 2014; 2015: 1-15.

Lipton SA. Neuronal protection and destruction by NO. Cell death and differ.1999; 6: 943–51.

Centeno JM, Orti M, Salom JB, Sick TJ, Perez- Pinzon MA. Nitric oxide is involved in anoxic preconditioning neuroprotection in rat hippocampal slices. Brain Res. 1999; 836: 62-69.

Kiziltepe U, Turan NND, Han U, Ulus T, Akar F. Resveratrol, a red wine polyphenol, protects spinal cord from ischemia- reperfusion injury. J Vasc Surg. 2004; 40: 138-45.

Kusum S, Chaudhary G, and Gupta YK. Protective effect of resveratrol against oxidative stress in middle cerebral artery occlusion model of stroke in rats. Life sciences. 2002; 71: 655-65.

De Zwart LL, Meerman JHN, Commandeur JNM, Vermeulen NPE. Biomarkers of free radical damage aplications in experimental animals and in humans. Free Radical Biology and Medicine. 1999; 26: 202-26.

Uchida K. Role of reactive aldehyde in cardiovasculer diseases. Free Radical Biology and Medicine. 2000; 28: 1685-96.

Mitsui Y, Schmelzer JD, Zollman PJ, Mitsui M, Tritschler HJ, Low PA. Alpha-lipoic acid provides neuroprotection from ischemia - reperfusion injury of peripheral nevre. J Neurol Sci. 1999; 163 : 11-6.

Gray C, Nukada H, Jackson DM, McMorran PD, Wu A, Ma F. Neuroprotective effects of nitrone radical scavenger S-PBN on reperfusion nerve injury in rats. Brain Res. 2003; 932: 179-85.

Mitsui Y, Okamoto K, Martin DP, Schmelzer JD, Low PA. The

expression of proinflammatory cytokine mRNA in the sciatic-tibial nerve of ischemia -reperfusion injury. Brain Research. 1999; 844: 192-5.

Published
2017-09-30
Section
Original Research