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Objective: Developmental dysplasia of the hip (DDH) is a sophisticated skeletal disease ranging from subluxation to entire dislocation of the hip as a result of missing growth of the acetabulum and femur. DDH clearly has a multifactorial biological etiology. No doubt, one of them is genetic changes. MicroRNAs (miRNAs) are 21-23 nt RNAs that show biological regulatory functions on a variety of basic physiological or pathological processes including bone formation. Although there have been many studies examining the relationship between bone metabolism and miRNAs, including miR-96, none of these studies, have been directed to the etiology of DDH.
Methods: In our study, we aimed to investigate the relationship between miR-96 expression level and DDH. In this study, peripheral blood samples of 50 DDH and 80 DDH-free individuals under one-year-old were analyzed to investigate the relationship between miR-96 expression level and DDH. Gene ontology (GO) and KEGG pathways enrichment analyses of miR-96 targeting genes were performed using available databases and R environment.
Results: We found that miR-96 level in DDH patients was approximately 3 times lower in comparison to healthy individuals. Gene ontology enrichments of hsa-mir-96 targeting genes underline the involvement of this miRNA in many biological processes including the bone homeostasis. Obtained results indicate that the expression level of miR-96 in the DDH group significantly decreased compared to the control group and this may be linked to the etiology of DDH.
Conclusion: Our work is the first study which aims to correlate the miR-96 level with DDH by investigating the miRNA level in plasma of patients and healthy individuals. In addition to our experimental data and enrichment analyses results for mir-96, considering the previous papers on miR-96 and osteogenesis, here we suggest that miR-96 may a role in the etiology of DDH.