The effect of radiotherapy on ether à-go-go potassium channel (Kv1.10) conductivity in DU145 prostate cancer cells

Abstract

ABSTRACT

Objective: Prostate cancer is the most common male urogenital system cancer in Turkey. After the lung cancer, it is the second most common cancer among all cancer types. Radiotherapy is one of the methods used in the treatment of prostate cancer. However, prostate cancer cells create resistance to radiotherapy for reasons that have not yet been fully explained. In this study, it is aimed to investigate the effect of radiotherapy on EAG1 potassium channel conductivity.

Methods: DU145 prostate cancer cell line was used in the study. Cells were divided into two groups as control and radiotherapy (RT). Cells in the RT group exposed to a single dose of 6 Gy RT. However, no treatment is taken on the cells in the control group. 120 minutes after application, EAG1 channel currents were recorded using the “whole cell patch-clamp technique” for both groups. Then, the current-voltage curves were drawn for each record and the curves were fit to a line equation. Channel conductivity was obtained by calculating the slope of the line.

Results: In terms of channel conductivity, statistically no significant difference was found between the control group and the RT group.

Conclusion: In conclusion, it has been observed that RT at 6 Gy dose has no effect on EAG1 channel currents, which are expressed at high levels in cancer cells and play an important role in cancer cell proliferation and migration. 

Keywords: Prostate cancer, radiotherapy, patch-clamp, oncochannels, EAG1 channels.