The Prophylactic and Protective Effects of Terfezia Claveryi Extracts on Ibuprofen Induced Oxidative Stress in Pregnant Rats

Authors

  • Mohammed Talat Abbas Department of Pharmaceutical Chemistry, College of Pharmacy, kerbala university , kerbala-Iraq

Abstract

Objective: This study aimed to investigate the prophylactic and protective roles of Terfezia Claveryi extracts on Ibuprofen induced oxidative stress in pregnant albino rats. Methods: Thirty pregnant rats randomly divided in to five experimental groups, each group consist of six pregnant rats. Group 1 (control group): without any treatment. Group 2 (Ibuprofen group): Given Ibuprofen orally by gastric tube at dose of 40 mg/kg/day for 20 days. Group 3 (Terfezia Claveryi group): Received Terfezia Claveryi extracts via gavage at 250 mg/kg every day dose. Group 4 (Ibuprofen +Terfezia Claveryi group): Received with Ibuprofen at dose of 40 mg/kg every day plus Terfezia Claveryi extracts 250 mg/kg /day by gastric tube along the experiment. Group 5 (Terfezia Claveryi + Ibuprofen): Received with 250 mg/kg every day of Terfezia Claveryi via gavage for two weeks before gestation (as a prophylactic) and then treated with Ibuprofen at the above-mentioned dose at zero day of gestation to twenty day of the experiment. Results: Ibuprofen increased serum AST, ALT, ALP activity, total bilirubin concentration, Liver tissue MDA levels and decreased liver tissue Catalase, Superoxide Dismutase and Glutathione Peroxidase. However, this modulation of the biological parameter is significantly ameliorated by the administration of the Terfezia Claveryi. Conclusion:In conclusion prophylactic and protective effect of Terfezia Claveryi against Ibuprofen induced oxidative stress was reported in pregnant rats.

References

Birben E, Sahiner UM, Sackesen C, Erzurum S, and Kalayci O, Oxidative Stress and Antioxidant Defense. WAO Journal 2012; 5:9–19.

Zarkovic N, et.al. Oxidative Stress and Antioxidants: Biological Response Modifiers of Oxidative Homeostasis in Cancer. PERIODICUM BIOLOGORUM.2010 ; 112, 4: 433–439.

Block G, Dietrich M, Norkus EP, Morrow JD, Hudes M, Caan B, Packer L. American journal of epidemiology. 2002; 156:274-285.

Green GA. Understanding NSAIDs: from aspirin to COX-2. Clin Cornerstone. 2001;3:50–9.

Burke A, Smyth EM, Fitzgerald GA. Analgesic-antipyretic agents; pharmacotherapy of gout. In: Brunton LL, Laxo JS, Parker KL, editors. Goodman & Gilman’s the pharmacologic basis of therapeutics. 11th ed. New York: McGraw-Hill; 2006.

Bradbury F. How important is the role of the physician in the correct use of a drug? An observational cohort study in general practice, International Journal of Clinical Practice, Supplement, 2004; 144:27-32.

Zoubair B, Azzahra LF, Fouzia H, Mohammed L, Brahim B and Noureddine B. Evaluation of Ibuprofen acid effect on oxidative stressed mice. Journal of Chemical and Pharmaceutical Research, 2016; 8(1):382-388.

Stancova V, Plhalova L, Blahova J, Zivna D, Bartoskova M, Siroka Z, Marsalek P, Svobodova Z. Effects of the pharmaceutical contaminants ibuprofen, diclofenac, and carbamazepine alone, and in combination, on oxidative stress parameters in early life stages of tench (Tinca tinca). Veterinarni Medicina, 62, 2017 (02): 90–97.

Higuchi K, Umegaki E, Watanabe T, Yoda Y, Morita E, Murano M, Tokioka S, Arakawa T. Present status and strategy of NSAIDs-induced small bowel injury, Journal of Gastroenterology, 2009; 44:879–88.

Kalaivani B , Thangapandiyan K and Vanithakumari G. Alleviation of Ibuprofen induced Nephrotoxicity by vitamin supplementations. Int. J. of Life Sciences, 2016; 4 (2): 203-206.

Walter H. Nonsteroidal Anti-Inflammatory Drugs and the KidneyPharmaceuticals 2010, 3, 2291-2321.

Halawa HM , El-Nefiawy NE. EVALUATION OF MELATONIN EFFECT ON IBUPROFEN INDUCED RENAL AND TESTICULAR TOXICITY IN ADULT MALE ALBINO RATS.2017;2(5):1-18.

Abatan MO, Lateef I, Taiwo VO. Toxic Effects of Non-Steroidal Anti-Inflammatory Agents in Rats. Afri J Biomed Res. 2006;9(3):219-23.

Casarica A Et.Al. A Purified Extract From Brown Truffles Of The Species Terfezia Claveryi Chatin And Its Antimicrobial Activity. Farmacia, 2016; 64: 2.

Saddiq AA, Danial EN. Assessment of phenolic content, free radical-scavenging capacity and antimicrobial activities of Truffle claveryi. Wulfenia J 2012; 19: 403-422. (2012).

Amna A. Saddiq JM. Youef AM.The Potential Antibacterial Role of Terfezia Claveryi Extract Against Immune-Inflammatory Disorder and Oxidative Damage Induced by Pseudomonas Aeruginosa in Rat Corneas. Romanian Biotechnological Letters. 2016; 21: 4.

Janakat S, Al‐Fakhiri S, Sallal AK. Phytotherapy Research.2004; 18:810-813.

Janakat S , Nassar M. Pakistan Journal of Nutrition.2010;9:52-56.

Al-Laith AA, Antioxidant components and antioxidant/antiradical activities of desert truffle (Tirmania nivea) from various Middle Eastern origins. Journal of Food Composition and Analysis.2010;23: 15–22

Jonah SA, Lucky LN, Cecilia NA. Evaluation of Toxicological Profile of Ibuprofen in Wistar Albino Rats. American Journal of Biomedical Sciences. 2014; 6: 32-40.

Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin.; 1957; 28:56-63.

Kind PR, King EJ. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol,1954; 7, 322-326.

Amour FF , Blood FR, Belden DA. The manual for laboratory work in mammalian Physiology. The University of Chicago Press, Chicago,1965 ;126-128.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurements with the folin phenol reagent. The Journal of Biological Chemistry.; 1951;193: 265-75.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry.1979;95: 351-8.

Worthington. V. Worthington Enzyme Manual, Lakewood, New Jersey.1975;5:20-21.

Beers RF, Jrand IW. Journal of Biological Chemistry.; 1952; 195:133-140 .

Flohéand L. Günzler WA .Methods in enzymology.1984;105: 114-120.

Begum, NA, Dewan ZF, Nahar N,Mamun MI. Bangladesh Journal of Pharmacology.;2006,1,16-20.

Ilango K. and Chitra V. Hepatproteactive and Antioxidant activities of Fruit Pulp of Limonia acidissima Linn. International Journal of Health Research. 2009; 2: 361‐367.

Kanchanaa N. Mohamed Sadiq . Hepatoprotective effect of Plumbago Zeylanica on paracetamol induced liver toxicity in rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2011; 3: 22-34.

Patel RK, Patel MM, Patel MP, Kanzaria NR, Vaghela KR, Patel NJ. Hepatoprotective activity of Moringa oleifera Lam. Fruit on isolated rat hepatocytes. Phcog mag. 2008; 4:118‐123.

Anitapal, Bhaskar B., Tanushree B., Manisha M., Kailash P. Hepatoprotective activity of Chenopodium Album Lbumlinn. Plant against paracetamol induced hepatic injury in rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2011; 3: 3-15.

Kalender S, Ogutcu A. Uzunhisarcikli M. Acikgoz F, Durak D, Ulusoy Y, Kalender Y. Diazinon-induced hepatotoxicity and protective effect of vitamin E on some biochemical indices and ultrastructural changes, Toxicol.2005;211:197-206.

Jayavelu1 A, Natarajan S, Sundaresan K, Devi B. Hepatoprotective Activity of Boerhavia Diffusa Linn. (Nyctaginaceae) against Ibuprofen Induced Hepatotoxicity in Wistar Albino Rats. International Journal of Pharma Research & Review, April 2013; 2:1-8.

Das RN, Mukherjee S, Sharma I. Alkaline Phosphatase Determinants of Liver Patients. JOP. J Pancreas 2018; 30; 19(1):18-23.

Li S et.al. The Role of Oxidative Stress and Antioxidants in Liver Diseases. Int. J. Mol. Sci. 2015;16: 26087–26124.

Maityi T et.al. Hepatoprotective activity of mikania scandens (L.) WILD.Against diclofenac sodium induced liver toxicity in rats. Asian J Pharm Clin Res, 2012;5: 185-189.

Anthony H. Kashou and Ashok Agarwal. Oxidants and Antioxidants in the Pathogenesis of HIV/AIDS. The Open Reproductive Science Journal, 2011, 3, 154-161.

Bayazit H , Selek S , Karababa IF , Cicek E , Aksoy N. Evaluation of Oxidant/Antioxidant Status and Cytokine Levels in Patients with Cannabis Use Disorder. Clinical Psychopharmacology and Neuroscience 2017;15(3):237-242.

Motor S et al. Evaluation of total antioxidant status, total oxidant status and oxidative stress index in patients with alopecia areata. Int J Clin Exp Med 2014;7(4):1089-1093.

Zhou L , Feng J , Zhang L, Kuang Y. Clinical significance of serum total oxidant/antioxidant status for the disease activity in active rheumatoid arthritis. Int J Clin Exp Pathol 2017;10(8):8895-8900.

Al-Laith AA. Antioxidant components and antioxidant/antiradical activities of desert truffle (Tirmania Nivea) from various Middle Eastern origins. J Food Compost Anal 2010; 23:15–22.

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Published

21.06.2019

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Original Research