The Effects of Sevoflurane and Desflurane on Hepatic Functions in Streptozotocin-Induced Diabetic Rats

Authors

  • Volkan Şıvgın Gazi University, Faculty of Medicine, Department of Anaesthesiology and Reanimation, Ankara, Turkey
  • Zerrin Özköse Şatırlar
  • Mustafa Arslan Gazi University, Faculty of Medicine, Department of Anaesthesiology and Reanimation, Ankara, Turkey
  • Mustafa Kavutçu
  • Mustafa Bilge
  • Gülen Akyol

Abstract

Objective: Diabetes mellitus (DM) is a common systemic disorder which is often encountered by anesthesiologists and associated with serious complications. Animal and clinical studies investigating the effects of volatile anesthetics and diabetes on organ functions are ongoing. In this study, we aimed to examine the histopathological and biochemical effects of sevoflurane and desflurane on hepatic functions in streptozotocin (STZ)-induced diabetic rats.

Materials and Methods: A total of 36 rats were randomly assigned into six groups: control group (Group C), diabetic control group (Group DC), desflurane group (Group D), sevoflurane group (Group S), diabetes-desflurane group (Group DD), and diabetes-sevoflurane group (Group DS). A single dose STZ 55 mg/kg was intraperitoneally injected to the diabetic groups. Diabetes was defined as having a blood glucose level of ≥250 mg/dL at 72 hours. At four weeks, desflurane 6% and sevoflurane 2% were administered in 100% oxygen over two hours. All anesthetized rats were administered intraperitoneal ketamine 100 mg/kg. Blood samples were collected from the abdominal aorta and all rats were sacrificed. Using the liver tissues, mean scores of injury (MSI) and the extent of Thiobarbituric Acid Reactive Substance (TBARS) were identified using and paraoxonase (PON) activities of anti-oxidant enzymes.

Results: Desflurane and sevoflurane increased MSI in the hepatic tissue; however, it did not reach a statistical significance. The MSI scores increased in diabetic rats compared to the control group. Desflurane and sevoflurane administration to the diabetic rats produced increased MSI scores, compared to the diabetic controls; however, it indicated no statistically significant difference. In the diabetic control group, TBARS increased, while PON decreased, compared to the control group. In the groups S and D, TBARS increased, while PON decreased, compared to the controls, suggesting no statistically significant difference. In the diabetic rats undergoing desflurane and sevoflurane administration, TBARS increased, whereas PON decreased.

Conclusion: Our study results show that desflurane and sevoflurane may lead to a mild to moderate hepatic injury in STZ-induced diabetic rats.

References

Reichle FM, Conzen PF. Halogenated inhalation anaesthetics. Best Pract Res Clin Anaesthesiol. 2003;17:29-46.

Alotaibi WM. Severe hepatic dysfunction after sevoflurane exposure. Saudi Med J. 2008;29(9):1344-1346.

Tung D, Yoshida EM, Wang CS, et al. Severe desflurane hepatotoxicity after colon surgery in an elderly patient. Can J Anaesth. 2005;52(2):133-136.

Guariguata L, Whiting D, Weil C, et al. The International Diabetes Federation diabetes atlas methodology for estimating global and national prevalence of diabetes in adults. Diabetes Res Clin Pract. 2011;94(3):322-332.

Satman I, Omer B, Tutuncu Y, et al. Twelve year trends in the prevalence and risk factors of diabetes and prediabetes in Turkish adults. Eur Epidemiol. 2013;28(2):169-180.

Tolman KG, Fonseca V, Dalpiaz A, et al. Spectrum of Liver Disease in Type 2 Diabetes and Management of Patients with Diabetes and Liver Disease. Diabetes Care 2007;30(3):734-743.

Rahimi R, Nikfar S, Larijani B, et al. A review on the role of antioxidants in the management of diabetes and its complications. Biomed Pharmacother. 2005;59(7):365-373.

Arslan M, Ozkose Z, Akyol G, et al. The age- and gender-dependent effects of desflurane and sevoflurane on rat liver. Exp Toxicol Pathol. 2010;62:35-43.

Zizek D, Ribnikara M, Zizek B, et al. Fatal subacute liver failure after repeated administration of sevoflurane anaesthesia. Eur J Gastroenterol Hepatol. 2010;22(1):112-115.

Soubhia AF, Lauz S, Montero EFS, et al. Effects of the Inhalational Anesthetics Halothane and Sevoflurane on an Experimental Model of Hepatic Injury. Rev Bras Anestesiol. 2011;61(5):591-603.

Srinivasan K, Ramarao P. Animal models in type 2 diabetes research: An overview. Indian J Med Res. 2007;125:451-472.

Arslan M, Comu FM, Isik B, Ozturk L, Kesimci E. Effect of dexmedetomidine on erythrocyte deformability during ischemia-reperfusion injury of liver in diabetic rats. Bratisl Med J 2012;113;687-691.

Lenzen S. The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia. 2008;51(2):216-226.

Taslidere E, Gul M, Elbe H, et al. The effects of caffeic acid phenethyl ester on streptozotocin-induced diabetic liver injury. Bratisl Med J. 2016;117(5):276-282.

Koksal GM, Sayilgan C, Aydin S, et al. The effects of sevoflurane and desflurane on lipid peroxidation during laparoscopic cholecystectomy. Eur J Anaesthesiol. 2004; 21:217-220.

Sivaci R, Kahraman A, Serteser M, et al. Cytotoxic effects of volatile anesthetics with free radicals undergoing laparoscopic surgery. Clin Biochem. 2006;39:293-298.

Ceylan BG, Yilmaz F, Eroglu F, et al. Oxidant and antioxidant of different anesthetic techniques. Propofol versus desflurane. Saudi Med J 2009;30:371-376.

Sheweita SA, Mashaly S, Newairy AA, et al. Changes in Oxidative Stress and Antioxidant Enzyme Activities in Streptozotocin-Induced Diabetes Mellitus in Rats: Role of Alhagi maurorum Extracts. Oxid Med Cell Longev. 2016;2016:5264064. doi: 10.1155/2016/5264064.

Hamadi N, Mansour A, Hassan MH, et al. Ameliorative Effects of Resveratrol on Liver Injury in Streptozotocin-Induced Diabetic Rats. J Biochem Mol Toxicol. 2012;26:384-392.

Aouacheri O, Saka S, Krim M, et al. The Investigation of the Oxidative Stress-Related Parameters in Type 2 Diabetes Mellitus. Can J Diabetes. 2015:39(1):44-49.

Litvinov D, Mahini H, Garelnabi M. Antioxidant and Anti-Inflammatory Role of Paraoxonase 1: Implication in Arteriosclerosis Diseases. N Am J Med Sci. 2012;4(11):523-532.

Wójcicka G, Jamroz-Wiśniewska A, Czechowska G, et al. The paraoxonase 1 (PON1), platelet-activating factor acetylohydrolase (PAF-AH) and dimethylarginine dimethylaminohydrolase (DDAH) activity in the metformin treated normal and diabetic rats. Eur J of Pharmacol. 2016;789:187-194.

Kopprasch S, Pıetzsch J, Kuhlısch E, et al. Lack of Association between Serum Paraoxonase 1 Activities and Increased Oxidized Low-Density Lipoprotein Levels in Impaired Glucose Tolerance and Newly Diagnosed Diabetes Mellitus. J Clin Endocrinol Metab. 2003;88:1711-1716.

Turillazzi E, D'Errico S, Neri M, et al. A Fatal case of fulminant hepatic necrosis following sevoflurane anesthesia. Toxicol Pathol. 2007;35:780-785.

Shingu K, Eger I, Johston B. Hepatic injury by anaesthetic agent rats. Anesth Analg. 1983;62:140-143.

Downloads

Published

2020-12-12

Issue

Section

Original Research