Effect of Resveratrol on Proinflammatory Gene Expression in Pancreas of Streptozotocin Induced Diabetic Rats
AbstractObjective: Inflammation and oxidative stress play an important role in the development of Diabetes mellitus (DM). The production amount of increased reactive oxygen types in ß-cells that are sensitive to oxidative stress cause degeneration in insulin release and insulin resistance in Type 2 DM. Streptozotocin(STZ) breaks down ß-cells with the accumulation of free radicals by inhibiting pancreas superoxide dismutase. And Resveratrol (RSV) has an inhibitory effect on the degenaration of pancreatic ß cells. In this study, we aimed to examine the impact of expressions in NF-κB, TNFα, IL-6, iNOS and COX2 genes in STZ-induced diabetes in pancreases of rats on the complications of DM. Methods: STZ-induced diabetes in pancreases of rats and RSV applied pancreatic tissues of rats after inducing diabetes are used. RNA isolation and cDNA synthesis are conducted for these tissues. mRNA expressions of NF-κB, TNFα, IL-6, iNOS and COX2 genes are realized with real-time PCR reaction and results are evaluated statistically. Results: No significant difference is observed between control, sham control and control+DMSO groups on COX2, iNOS, NF-κB, TNFα and IL-6 in mRNA level. When control and diabetes groups are compared, it is observed that mRNA levels of COX2, iNOS, NF-κB, TNFα and IL-6 genes increased in mRNA expression levels of target genes in diabetes group. This presents a statistical meaning between two groups. And when diabetes and diabetes+RSV groups are compared, decrease in COX2, iNOS, NF-κB, TNFα and IL-6 genes are observed in mRNA levels. It is observed that this decrease is not statistically meaningful. Conclusion: Data obtained shows that there is decrease in expression levels of inflammatory genes in diabetic rats and RSV application has no statistically significant effect on expression levels of these genes. From this point of view it can be said that only RSV application cannot change the expression levels of inflammatory genes associated with diabetes.
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