Effects of Lipopolysaccharides of Pseudomonas Aeruginosa and Aqueous Extract of Ginkgo Biloba, Ginkgoaceae, on Cellular Immune Response in Mice Balb/c

  • Najeeb Mohammed Hussein University of Anbar
  • Mustafa Nadhim Owaid Ministry of Education
  • Ban Hamid Khalaf University of Anbar
Keywords: LPS, immunology, herbal extract, in vivo, maidenhair tree.


In this study, lipopolysaccharides (LPS) of Pseudomonas aeruginosa IAN5 isolate and aqueous extract of Ginkgo biloba leaves were used to investigate the cellular immunity in mice Balb/c (in vivo). Some parameters were used to achieve this study, are percentages of polymorphonuclear neutrophils (PMNs), phagocytosis coefficient of PMNs at different time periods 30, 60, 90 and 120 minutes, Formazan granules formation in them, and migration inhibition factor (MIF). The immunization of mice with the LPS antigen affected delay-type hypersensitivity and increased activity of PMNs in Candida sp. and reduced NBT, while inhibited migration of PMNs. The immunization did not affect the macrophages of PMNs. The injected treatment with LPS and Ginkgo biloba extract showed the best results significantly (p<0.05) compared to LPS and control treatments, whereas the Ginkgo biloba extract alone showed no significant differences (p<0.05).

Author Biographies

Najeeb Mohammed Hussein, University of Anbar
Department of Biology, College of Science
Mustafa Nadhim Owaid, Ministry of Education
Department of Heet Education, General Directorate of Education in Anbar
Ban Hamid Khalaf, University of Anbar
College of Dentistry


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Original Research