The Effect of Dexmedetomidine on Ischemia Reperfusion Injury in Myocard of Rat
AbstractObjective: The aim of this study was to evaluate the effect of dexmedetomidine (100µg/kg-ip) on ischemia reperfusion (IR) injury in myocard of rats. Methods: Twenty-four Wistar Albino rats were separated into four groups. There were four experimental groups (Group C (Control; n=6), Group IR (ischemia-reperfusion, n=6), Group D (Dexmedetomidine; n=6), underwent left thoracotomy and received ip dexmedetomidine without ischemia and reperfusion and Group IR-D (IR-Dexmedetomidine; n=6) was administrated 100µg/kg dexmedetomidine via ip route 30 minutes before ligating the left coronary artery. A small plastic snare was threaded through the ligature and placed in contact with the heart. To produce IR, a branch of the left coronary artery was occluded for 30 min followed by two hours of reperfusion. However, after the above procedure, the coronary artery was not occluded or reperfused in the control rats. At the end of the study, myocard tissue was obtained for biochemical, histochemical and immunohistochemical determination/ analyses . Results: Myonecrosis, cell infiltration and edema were significantly higher in the IR group than in the C and D groups. In the IR-D group, myonecrosis, cell infiltration and edema were significantly lower than in the IR group.TBARS levels were found to be significantly higher in the IR group than in the C and D groups.TBARSlevels in the IR-D group were found to be significantly lower than in the IR group.SOD enzyme activity was found to be significantly lower in the IR group than in the C group. In the IR-D group, SOD enzyme activity was found to be significantly higherthan the IR group. Conclusion: Dexmedetomidine removed degenerative effects after ischemia reperfusion in ischemia reperfusion group and we may conclude that dexmedetomidine may have regenerative effects on IR injury .
Zimmerman BJ, Granger DN. Reperfusion injury. Surg Clin North Am 1992;72:65–83.
Loft S, Larsen PN, Rasmussen A, Fischer-Nielsen A, Bondesen S, Kirkegaard P, et al. Oxidative DNA damage after transplantation of the liver and small intestine in pigs. Transplantation 1995;59:16–20.
Guo J, Wang SB, Yuan TY, Wu YJ, Yan Y, Li L, e al. Coptisine protects rat heart against myocardial ischemia/reperfusion injury by suppressing myocardial apoptosis and inflammation. Atherosclerosis 2013;231:384-91.
Hearse DJ,Humphrey SM,ChainEB.Abruptre oxygenation of the anoxic potassium arrested perfused rat heart: a study of myocardial enzyme release.JMol CellCardiol 1973; 5:395- 407.
GracePA.Ischaemia-reperfusion injury. Br JSurg 1994;81:637-47.
Hergenç G.Complementary system’s role is in atherosklerosis. ArchTurk Soc Cardiol 2004; 32:28-37.
Wang Y, Ji M, Chen L, WuX, Wang L. Breviscapine reduces acute lung injury induced by left heart ischemic reperfusion in rats by inhibiting the expression of ICAM-1 and IL-18. Exp Ther Med 2013;6:1322-6.
McCutcheon CA, Orme RM, Scott DA, et al. A comparison of dexmedetomidine versus conventional therapy for sedation and hemodynamic controlduring carotid endarterectomy performed under regional anesthesia. AnesthAnalg 2006;102:668-75.
Ramsay MA, Luterman DL. Dexmedetomidine as a total intravenous anesthetic agent. Anesthesiology 2004;101:787-90.
Maier CM, Sun GH, Kunis DM, et al. Neuroprotection by the N-methyl-D-aspartate receptor antagonist CGP 40116: In vivo and in vitro studies. J Neurochem 1995;65:652-9.
Hoffman WE, Kochs E, Werner C, et al. Dexmedetomidine improves neurologic outcome from incomplete ischemia in the rat. Reversal by the alpha 2- adrenergic antagonist atipamezole. Anesthesiology 1991;75:328-32.
Durak I, Canbolat O, Kavutcu M, Öztürk HS, Yurtarslanı Z. Activities of total, cytoplasmic and mihochondrial superoxide dismutase enzymes in sera and pleural fluids from patient with lung cancer. J Clin Lab Anal 1996; 10: 17-20.
Aebi H. Catalase. In: H.U.Bergmeyer (Ed): Methods of Enzymatic Analysis, Academic Press , New York and London, 1974; pp.673-7.
Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130– 9.
Van Ye TM, Roza AM, Pieper GM, Henderson J Jr, Johnson JP, Adams MB. Inhibition of intestinal lipid peroxidation does not minimize morphological damage. J Surg Res 1993; 55:553-8.
Kocoglu H, Karaaslan K, Gonca E, et al. Preconditioning effects of dexmedetomidine on myocardial ischemia/reperfusion injury in rats. Curr Ther Res Clin Exp 2008;69:150-8.
Kabukçu HK, Sahin N, Temel Y, Titiz TA. Hemodynamics in coronary artery bypass surgery: Effects of intraoperative dexmedetomidine administration. Anaesthesist 2011;60:427-31.
Mangano DT, Browner WS, Hollenberg M, et al, for the Study of Perioperative IschemiaResearch Group. Association of perioperative myocardial ischemia with cardiac morbidity and mortality in men undergoing noncardiac surgery. N EnJff Ned 1990;323:1781-8.
Wheeler CR,Salzman JA, Elsayed NM, Omaye ST, Korte DW Jr, Automated assays for superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activity. Anal Biochem 1990;184: 193-9.
Mates JM, Perez-Gomez C, Castro IN. Antioxidant enzymes and human disease. Clin Biochem 1999; 32:595-603.
Yerer MB, Yapislar H, Aydogan S, Yalcin O, Baskurt O. Lipid peroxidation and deformability of red blood cells in experimental sepsis in rats: The protective effects of melatonin. ClinHemorheol Microcirc 2004; 30: 77-82.
Johnson F, Giulivi C. Superoxide dismutases and their impactupon human health. Mol Aspects Med 2005; 26: 340-52.
Yagmurdur MC, Ozdemir A, Topaloglu S, Kilinc¸ K, Ozenc¸ A.Effects of alpha tocopherol and verapamil on liver and smallbowel following mesenteric ischemia-reperfusion. Turk J Gastroenterol 2002; 13: 40-6.
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