Genotoxic Studies Performed After Radiofrequency Radiation Exposure

Authors

  • Neslihan Gürbüz Gazi Üniversitesi Tıp Fakültesi Gazi Hastanesi Biyokimya ve Hormon Laboratuvarı Beşevler- Ankara
  • Bahriye Sırav
  • Nesrin Seyhan

Abstract

Objective: With the development of technology human beings are increasingly under the exposure of electromagnetic fields, mainly radio-frequency radiation (RFR) from wireless technologies, mobile phones, base stations etc. There are many genotoxic effects, i.e. DNA and chromosome change studies related RFR exposures. There is still some uncertainty, no definitive conclusions have been reached so far. The aim of present study is to evaluate the genotoxicity studies about RFR exposure reported from 1989 to 2016. Methods: The PubMed database from 1989 to 2016 was searched for “radio-frequency radiation” and “genotoxicity tests”. Genotoxicity studies performed under RFR exposure were selected and classified as reported significant effects and reported no significant effect. Results: There were 53 genotoxic effects of RFR studies totally and 19 of them indicated genotoxic effects (35,8 %) and 34 of total studies reported no significant effect (64,2 %). Conclusion: It is apparent that there is no consistent pattern that RFR exposure could induce genetic damages. However, one can conclude that under certain conditions of exposure, RFR could be genotoxic. Generally the genotoxic effect of short-term exposure to RFR have been studied up to date. Long-term exposure to RFR is increasing in the environment. So, long-term effect of repeated exposure to RFR should also be studied by taking into consideration the DNA repair processes in order to identify the biological mechanisms involved.

Author Biography

Neslihan Gürbüz, Gazi Üniversitesi Tıp Fakültesi Gazi Hastanesi Biyokimya ve Hormon Laboratuvarı Beşevler- Ankara

Toxicology

References

BioInitiative 2012, A Rationale for Biologically-based Exposure Standards for Low-Intensity Electromagnetic Radiation, section 6.

Verschaeve L, Juutilainen J, Lagroye I, Miyakoshi J, Saunders R, de Seze R, et al. In vitro and in vivo genotoxicity of radiofrequency fields. Mutat Res. 2010; 705:252-68.

Gandhi G, Kaur G, Nisar U. A cross-sectional case control study on genetic damage in individuals residing in the vicinity of a mobile phone base station. Electromagn Biol Med. 2015; 34:344-54.

Atlı Şekeroğlu Z, Akar A. Evaluation of the cytogenotoxic damage in immature and mature rats exposed to 900 MHz radiofrequency electromagnetic fields. Int J Radiat Biol. 2013; 89: 985-92.

Liu C, Duan W, Xu S, Chen C, He M, Zhang L. et al. Exposure to 1800 MHz radiofrequency electromagnetic radiation induces oxidative DNA base damage in a mouse spermatocyte-derived cell line. Toxicol Lett. 2013; 218:2-9.

Jiang B, Nie J, Zhou Z, Zhang J, Tong J, Cao Y. Adaptive response in mice exposed to 900 MHz radiofrequency fields: primary DNA damage. PLoS One. 2012; 7:e32040.

Çam ST, Seyhan N. Single-strand DNA breaks in human hair root cells exposed to mobile phone radiation. Int J Radiat Biol. 2012;88:420- 4.

Karaca E, Durmaz B, Aktug H, Yildiz T, Guducu C, Irgi M. et al. The genotoxic effect of radiofrequency waves on mouse brain. Neurooncol. 2012; 106:53-8.

Sannino A, Zeni O, Sarti M, Romeo S, Reddy SB, Belisario MA et al. Induction of adaptive response in human blood lymphocytes exposed to 900 MHz radiofrequency fields: influence of cell cycle. Int J Radiat Biol. 2011;87:993-9.

Lee JW, Kim MS, Kim YJ, Genotoxic effects of 3 T magnetic resonance imaging in cultured human lymphocytes. Bioelectromagnetics. 2011;32:535-42.

Zhijian C, Xiaoxue L, Yezhen L, Shijie C, Lifen J, Jianlin L. et al. Impact of 1.8-GHz radio-frequency radiation (RFR) on DNA damage and repair induced by doxorubicin in human B-cell lymphoblastoid cells. Mutat Res. 2010; 695:16-21.

Yao K, Wu W, Wang K, Ni S, Ye P, Yu Y. et al. Electromagnetic noise inhibits radiofrequency radiation-induced DNA damage and reactive oxygen species increase in human lens epithelial cells. Mol Vis. 2008;14:964-9.

Yao K, Wu W, Yu Y, Zeng Q, He J, Lu D, Wang K. et al. Effect of superposed electromagnetic noise on DNA damage of lens epithelial cells induced by microwave radiation. Invest Ophthalmol Vis Sci. 2008 ;49: 2009-15.

Schwarz C, Kratochvil E, Pilger A, Kuster N, Adlkofer F, Rüdiger HW. et al. Radiofrequency electromagnetic fields (UMTS, 1,950 MHz) induce genotoxic effects in vitro in human fibroblasts but not in lymphocytes. Int Arch Occup Environ Health. 2008;81:755-67.

Trosic I, Busljeta I. Erythropoietic dynamic equilibrium in rats maintained after microwave irradiation. Exp Toxicol Pathol. 2006;57:247-51.

Baohong W, Jiliang H, Lifen J, Deqiang L, Wei Z, Jianlin L. et al. Studying the synergistic damage effects induced by 1.8 GHz radio-frequency field radiation (RFR) with four chemical mutagens on human lymphocyte DNA using comet assay in vitro. Mutat Res. 2005; 578:149-57.

Busljeta I, Trosic I, Milkovic-Kraus S. Erythropoietic changes in rats after 2.45 GJz nonthermal irradiation. Int J Hyg Environ Health. 2004; 207:549-54.

Trosic I, Busljeta I, Modlic B. Investigation of the genotoxic effect of microwave irradiation in rat bone marrow cells: in vivo exposure. Mutagenesis. 2004;19:361-4.

Trosic I, Busljeta I, Kasuba V, Rozgaj R. Micronucleus induction after whole-body microwave irradiation of rats.Mutat Res. 2002; 521:73-9.

Garaj-Vrhovac V. Micronucleus assay and lymphocyte mitotic activity in risk assessment of occupational exposure to microwave radiation. Chemosphere. 1999; 39:2301-12.

Zhu K, Lv Y, Cheng Q, Hua J, Zeng Q. Extremely Low Frequency Magnetic Fields Do Not Induce DNA Damage in Human Lens Epithelial Cells In Vitro. Anat Rec (Hoboken). 2016 ;299:688-97.

Kumar G, McIntosh RL, Anderson V, McKenzie RJ, Wood AW. A genotoxic analysis of the hematopoietic system after mobile phone type radiation exposure in rats. Int J Radiat Biol. 2015;91:664-72.

Speit G, Gminski R, Tauber R. Genotoxic effects of exposure to radiofrequency electromagnetic fields (RF-EMF) in HL-60 cells are not reproducible. Mutat Res. 2013; 755:163-6.

Vijayalaxmi, Reddy AB, McKenzie RJ, McIntosh RL, Prihoda TJ, Wood AW. Incidence of micronuclei in human peripheral blood lymphocytes exposed to modulated and unmodulated 2450 MHz radiofrequency fields. Bioelectromagnetics 2013; 34:542-8.

Waldmann P, Bohnenberger S, Greinert R, Hermann-Then B, Heselich A, Klug SJ, et al. Influence of GSM signals on human peripheral lymphocytes: study of genotoxicity. Radiat Res.2013; 179:243-53.

Jiang B, Zong C, Zhao H, Ji Y, Tong J, Cao Y. Induction of adaptive response in mice exposed to 900MHz radiofrequency fields: application of micronucleus assay. Mutat Res. 2013; 751:127-9.

Ros-Llor I, Sanchez-Siles M, Camacho-Alonso F, Lopez-Jornet P. Effect of mobile phones on micronucleus frequency in human exfoliated oral mucosal cells. Oral Dis. 2012;18:786-92.

Trosić I, Pavicić I, Milković-Kraus S, Mladinić M, Zeljezić D. Effect of electromagnetic radiofrequency radiation on the rats' brain, liver and kidney cells measured by comet assay. Coll Antropol.2011; 35:1259-64.

Sannino A, Di Costanzo G, Brescia F, Sarti M, Zeni O, Juutilainen J. et al. Human fibroblasts and 900 MHz radiofrequency radiation: evaluation of DNA damage after exposure and co-exposure to 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5h)-furanone (MX). Radiat Res. 2009; 171:743-51.

Zhijian C, Xiaoxue L, Yezhen L, Deqiang L, Shijie C, Lifen J.et al.Influence of 1.8-GHz (GSM) radio-frequency radiation (RFR) on DNA damage and repair induced by X-rays in human leukocytes in vitro. Mutat Res. 2009; 677:100-4.

Luukkonen J, Hakulinen P, Mäki-Paakkanen J, Juutilainen J, Naarala J. Enhancement of chemically induced reactive oxygen species production and DNA damage in human SH-SY5Y neuroblastoma cells by 872 MHz radiofrequency radiation. Mutat Res. 2009;662: 54-8.

Kim JY, Hong SY, Lee YM, Yu SA, Koh WS, Hong JR. et al. In vitro assessment of clastogenicity of mobile-phone radiation (835 MHz) using the alkaline comet assay and chromosomal aberration test. Environ Toxicol. 2008; 23:319-27.

Zeni O, Schiavoni A, Perrotta A, Forigo D, Deplano M, Scarfi MR. Evaluation of genotoxic effects in human leukocytes after in vitro exposure to 1950 MHz UMTS radiofrequency field. Bioelectromagnetics. 2008; 29:177-84.

Juutilainen J, Heikkinen P, Soikkeli H, Mäki-Paakkanen J. Micronucleus frequency in erythrocytes of mice after long-term exposure to radiofrequency radiation. Int J Radiat Biol. 2007; 83:213-20.

Chauhan V, Mariampillai A, Kutzner BC, Wilkins RC, Ferrarotto C, Bellier PV. et al. Evaluating the biological effects of intermittent 1.9 GHz pulse-modulated radiofrequency fields in a series of human-derived cell lines. Radiat Res. 2007; 167:87-93.

Lixia S, Yao K, Kaijun W, Deqiang L, Huajun H, Xiangwei G. et al. Effects of 1.8 GHz radiofrequency field on DNA damage and expression of heat shock protein 70 in human lens epithelial cells. Mutat Res. 2006; 602:135-42.

Scarfì MR, Fresegna AM, Villani P, Pinto R, Marino C, Sarti M. et al. Exposure to radiofrequency radiation (900 MHz, GSM signal) does not affect micronucleus frequency and cell proliferation in human peripheral blood lymphocytes: an interlaboratory study. Radiat Res. 2006; 165:655-63.

Stronati L, Testa A, Moquet J, Edwards A, Cordelli E, Villani P. et al. 935 MHz cellular phone radiation. An in vitro study of genotoxicity in human lymphocytes. Int J Radiat Biol. 2006; 82:339-46.

Verschaeve L, Heikkinen P, Verheyen G, Van Gorp U, Boonen F, Vander Plaetse F. et al. Investigation of co-genotoxic effects of radiofrequency electromagnetic fields in vivo. Radiat Res. 2006; 165:598-607.

Maes A, Van Gorp U, Verschaeve L. Cytogenetic investigation of subjects professionally exposed to radiofrequency radiation. Mutagenesis. 2006; 21:139-42.

Sakuma N, Komatsubara Y, Takeda H, Hirose H, Sekijima M, Nojima T. et al. DNA strand breaks are not induced in human cells exposed to 2.1425 GHz band CW and W-CDMA modulated radiofrequency fields allocated to mobile radio base stations. Bioelectromagnetics. 2006; 27:51-7.

Nikolova T, Czyz J, Rolletschek A, Blyszczuk P, Fuchs J, Jovtchev G. et al. Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells. FASEB J. 2005; 19:1686-8.

Diem E, Schwarz C, Adlkofer F, Jahn O, Rüdiger H. Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro. Mutat Res. 2005; 583:178-83.

Hook GJ, Zhang P, Lagroye I, Higashikubo R, Moros EG, Straube WL et al. Measurement of DNA damage and apoptosis in Molt-4 cells after in vitro exposure to radiofrequency radiation Radiat Res. 2004;161:193-200.

Bisht KS, Moros EG, Straube WL, Baty JD, Roti JL. The effect of 835.62 MHz FDMA or 847.74 MHz CDMA modulated radiofrequency radiation on the induction of micronuclei in C3H 10T(1/2) cells. Radiat Res. 2002;157:506-15.

Vijayalaxmi, Pickard WF, Bisht KS, Prihoda TJ, Meltz ML, LaRegina MC. et al. Micronuclei in the peripheral blood and bone marrow cells of rats exposed to 2450 MHz radiofrequency radiation. Int J Radiat Biol. 2001;77:1109-15.

Li L, Bisht KS, LaGroye I, Zhang P, Straube WL, Moros EG. et al. Measurement of DNA damage in mammalian cells exposed in vitro to radiofrequency fields at SARs of 3-5 W/kg. Radiat Res. 2001;156:328-32.

Vijayalaxm, Leal BZ, Szilagyi M, Prihoda TJ, Meltz ML Primary DNA damage in human blood lymphocytes exposed in vitro to 2450 MHz radiofrequency radiation. Radiat Res. 2000; 153:479-86.

Maes A, Collier M, Van Gorp U, Vandoninck S, Verschaeve L. Cytogenetic effects of 935.2-MHz (GSM) microwaves alone and in combination with mitomycin C. Mutat Res. 1997; 393:151-6.

Vijayalaxmi, Frei MR, Dusch SJ, Guel V, Meltz ML, Jauchem JR. Frequency of micronuclei in the peripheral blood and bone marrow of cancer-prone mice chronically exposed to 2450 MHz radiofrequency radiation. Radiat Res. 1997; 147:495-500.

Meltz ML, Eagan P, Erwin DN. Absence of mutagenic interaction between microwaves and mitomycin C in mammalian cells. Environ Mol Mutagen. 1989; 13:294-303.

Gurbuz N, Sirav B, Colbay M, Yetkin I, Seyhan N. No genotoxic effect in exfoliated bladder cells of rat under the exposure of 1800 and 2100 MHz radio-frequency radiation. Electromagn Biol Med. 2014;33:296-301.

Gurbuz N, Sirav B, Yuvaci HU, Turhan N, Coskun ZK, Seyhan N. Is there any possible genotoxic effect in exfoliated bladder cells of rat under the exposure of 1800 MHzGSM-like modulated radio-frequency radiation (RFR)? Electromagn Biol Med. 2010;29:98-104.

Moulder JE, Foster KR, Erdreich LS, McNamee JP. Mobile phones, mobile phone base stations and cancer: a review. Int J Radiat Biol. 2005;8 1:189-203.

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Published

15.03.2018

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